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Thiosulfate-citrate-bile salts-sucrose agar or TCBS agar is a type of selective agar culture plate that is used in microbiology laboratories to isolate ''Vibrio'' spp. TCBS Agar is highly selective for the isolation of V. cholerae and V. parahaemolyticus as well as other vibrios. TCBS agar contains high concentrations of Sodium thiosulfate and Sodium citrate to inhibit the growth of Enterobacteriaceae. Inhibition of Gram-positive bacteria is achieved by the incorporation of ox gall, which is a naturally occurring substance containing a mixture of bile salts, and sodium cholate, a pure bile salt. Sodium thiosulfate also serves as a sulfur source and, in combination with ferric citrate, detects hydrogen sulfide production. Saccharose (sucrose) is included as a fermentable carbohydrate for the metabolism of vibrios. The alkaline pH of the medium enhances the recovery of V. cholerae and inhibits the growth of others. Thymol blue and bromothymol blue are included as indicators of pH changes. == Formula == ;Approximate formula per liter * Yeast extract 5.0g * Proteose Peptone 10.0g * Sodium thiosulfate 10.0g * Sodium citrate 10.0g * Ox gall 5.0g * Sodium cholate 3.0g * Saccharose 20.0g * Sodium chloride 10.0g * Ferric citrate 1.0g * Bromothymol blue 0.04g * Thymol blue 0.04g * Agar 15.0g 抄文引用元・出典: フリー百科事典『 ウィキペディア（Wikipedia）』 ■ウィキペディアで「Thiosulfate-citrate-bile salts-sucrose agar」の詳細全文を読む スポンサード リンク
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